Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit
On demandThermo Scientific™ Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit
Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Features
- Ready to use—carefully formulated and prefiltered 1X solutions
- Three binding buffers—optimized for Protein A, Protein G or Protein A/G
- Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral)
- Versatile—elution buffers are appropriate for antibody-antigen affinity purification
Contents
Sufficient For: Binding and near-neutral elution of antibodies with Protein A or G
- Pierce Gentle Ag/Ab Binding Buffer: 100 mL
- Pierce Gentle Ag/Ab Elution Buffer: 100 mL
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