Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA

Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA

The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing N-Acetyl glucosamine (GlcNAc) groups or sialic acid. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features

• High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
• Fast—glycoprotein purification in less than an hour
• Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
• Robust—lectin does not leach from resin when processing sample
• Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
• Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Contents

• WGA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes