Thermo Scientific™ Pierce™ Glycoprotein Staining Kit

Thermo Scientific™ Pierce™ Glycoprotein Staining Kit

The Pierce Glycoprotein Staining Kit is a convenient, fast, and sensitive colorimetric kit for staining glycosylated proteins in polyacrylamide gels or nitrocellulose membranes using the periodic acid-Schiff (PAS) method.

Features

• Specific for glycoproteins—PAS method specifically detects glycosylated proteins with sialic acid and other oxidizable carbohydrate groups
• Protocol—the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours
• Colorimetric—glycoproteins are detected as magenta bands against a faint pink or colorless gel background
• Qualitative staining—detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625 ng avidin and 0.16 µg horseradish peroxidase per band
• Controls included—complete kit includes positive and negative control proteins (HRP and STI, respectively).

Contents

• Oxidation Reagent, 2.5 g powder to make 250 mL
• Reduction Reagent, 1.25 g powder to make 250 mL
• Positive Control (Horseradish Peroxidase), 1 mg
• Negative Control (Soybean Trypsin Inhibitor), 1 mg
• Glycoprotein Stain, 250 mL