Thermo Scientific Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible, 500 mL

Thermo Scientific Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible, 500 mL

Pierce high-capacity EDTA-compatible Ni-IMAC resin is designed for the purification of over-expressed His-tagged proteins from cell culture media. The matrix is a 6% crosslinked agarose with optimal properties for batch, gravity column, and research/pilot scale FPLC column purifications. The resin has high binding capacity (up to 80 mg of protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features

• Chelator stable—use up to 20 mM EDTA and DTT with no loss in performance
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• High binding capacity—>80 mg/mL binding capacity for His-tagged recombinant proteins
• Reusable—can be regenerated up to 10 times using the CIP protocol
• Liquid suspension
• Matrix: 6% crosslinked agarose
• Particle Size: 50 - 150 µm

Contents

• Settled resin, supplied as 1000 mL 50% slurry: 500 mL