Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL), 10000 Units

Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL), 10000 Units

Thermo Scientific Pierce Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.

Features

• Effective—HRV 3C is designed to remove purification tags from your protein of interest
• High activity at 4°C—activity is ≥2000U/mg control protein; one unit will cleave 0.1 mg control protein at 4°C after 16h
• Flexible—dual-tagged enzyme is compatible with various columns and buffers

Contents

• HRV 3C Protease (2 units/µL), 5000µL (1mg/mL)
• GST-Syk Protein, Positive Control, 25µL (1mg/mL)
• 10X HRV 3C Reaction Buffer, 10mL