Thermo Scientific™ Pierce™ Iodination Reagent (1,3,4,6-tetrachloro-3α,6α-diphenyl-glycoluril)

Thermo Scientific™ Pierce™ Iodination Reagent (1,3,4,6-tetrachloro-3α,6α-diphenyl-glycoluril)

Thermo Scientific Pierce Iodination Reagent is 1,3,4,6-tetrachloro-3a,6a-diphenyl-glycoluril, an oxidizing agent that efficiently activates iodine for spontaneous incorporation into protein tyrosyl groups.

Features

• Extremely stable and virtually insoluble in water (Fraker and Smith, 1978)
• Solubility in chloroform permits 'plating' aliquots of the reagent on the walls of the glass or plastic iodination vessels; plated vessels can be prepared in advance and stored in a dessicator
• Allows rapid iodination of proteins in an aqueous reaction mixture containing radioactive Na125I
• Side reactions are negligible
• No reducing reagent is required, because reactions are terminated by simply decanting the reaction solution away from the plated IODO-GEN Iodination Reagent
• Specific activities of 1 x 105 cpm of 125I per microgram of protein and labeling efficiencies of 60% are easily achieved
• Can be used to iodinate phenolic groups on crosslinkers or other protein modification reagents either before, or after, a protein, peptide or cell surface is reacted with the modification reagent
• Small amounts of protein can be iodinated (Tolan, et al., 1980)
• More efficient than Chloramine-T or Lactoperoxidase (Salacinski, 1981)
• Iodinate phenolic groups on crosslinking reagents (see list of compatible reagents)