Thermo Scientific™ Pierce™ Lys-C Protease, MS Grade, 100 µg
On demandThermo Scientific™ Pierce™ Lys-C Protease, MS Grade, 100 µg
Thermo Scientific™ Pierce™ Lys-C Protease, MS Grade is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
This is a mass spectrometry (MS)-grade serine protease isolated from Lysobacter enzymogenes. Lys-C protease has high activity and specificity for lysine residues, resulting in larger peptides and less sample complexity than trypsin (i.e., fewer peptides). Unlike trypsin, Lys-C protease can cleave lysines followed by prolines, making it ideal for sequential protein digestion followed by trypsin to decrease missed cleavages. These unique Lys-C protease properties ensure high digestion efficiency when used alone or followed by tryptic digestion. Additionally, Lys-C prototypic peptides typically have higher charge states, making it an enzyme of choice for use with ETD fragmentation.
Features
- Enhanced digestion—use in tandem with trypsin to decrease tryptic missed cleavages
- Increased sequence coverage—better protein characterization results from overlapping peptides with complementary chromatographic, ionization, and fragmentation properties
- Versatility—effective enzyme activity under highly denaturing conditions (e.g., 8 M urea)
- C-terminal lysine cleavage specificity—at least 90% for a complex protein sample
- Stability—provided in a lyophilized format