Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit

Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit

The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with the Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.

Features

• Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
• Functional – cultured fibroblasts express the appropriate biochemical markers

Contents

Sufficient For: Isolation of MEFs from 50 embryonic mouse somatic tissues

• DMEM for Primary Cell Isolation, 500 mL
• MEF Isolation Enzyme (with papain), 5 vials
• Hanks' Balanced Salt Solution (HBSS without Ca2+, Mg2+), 500 mL