Thermo Scientific™ Pierce™ PNPP Substrate Kit

Thermo Scientific™ Pierce™ PNPP Substrate Kit

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Our Phosphatase Substrate Kit contains PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Part No. 34064) is also provided individually as a 5X concentrate. The Pierce formulation has an optimal and consistent pH and it is stable even at a 1X concentration.

Features

• One-component alkaline phosphatase (AP) substrate

• Absorbance maximum of 405nm

• Develops a yellow hue in solution upon reaction with AP

• Safer to use—contains a non-mercury, non-azide preservative

PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.

• Product Line: Pierce™
• Description: Pierce PNPP Substrate Kit
• Product Type: ELISA Substrate
• Store buffer at 4°C and PNPP tablets at -20°C.

Content

Sufficient For: 5mL of typical 1X substrate solution per tablet (525 mL total; approx. 5250 wells at 100 µL per well)

• Diethanolamine Buffer, 225 mL
• PNPP Tablets, 105 tablets (5 mg/tablet)