Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns, 1.8 K MWCO, 5 mL

Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns, 1.8 K MWCO, 5 mL

Thermo Scientific Pierce 1.8K MWCO (molecular-weight cutoff) Polyacrylamide Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular-weight buffer salts and reagents.

Pierce 1.8K MWCO Polyacrylamide Desalting Columns are pre-packed with porous polyacrylamide beads and provide for buffer-exchange and desalting of proteins and other macromolecular samples based on a molecular-weight cutoff (MWCO) of 1800. Unlike other size-exclusion media, polyacrylamide resin is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The resin is very hydrophilic, thereby minimizing undesirable binding interactions between the resin and sample molecules. Contamination with low molecular weight sugars (which may occur with crosslinked dextran resin) is not a concern when using polyacrylamide resin. Oxidizing agents can be removed without destroying the support. This resin is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2-10 is recommended at room temperature. The polyacrylamide resin can also be autoclaved at pH 5.5-6.5 for 30 minutes at 120°C.

Features

• Stable in water, salt solutions, organic solvents and alkaline or acidic conditions
• Excellent flow properties
• Heat-stable
• MWCO: 1800 kDa