Thermo Scientific™ Pierce™ Protein A Chromatography Cartridges, 1 mL

Thermo Scientific™ Pierce™ Protein A Chromatography Cartridges, 1 mL

The Thermo Scientific™ Pierce™ Chromatography Cartridges Protein A are convenient, ready-to-use pre-packed devices for the isolation and purification of polyclonal and monoclonal antibodies. These cartridges are effective for routine affinity purification of polyclonal and monoclonal IgG from serum, ascites and cell culture supernatant.

Pierce Protein A Plus Agarose consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features

• Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34 mg human IgG/mL resin (approx. 16 to 17 mg mouse IgG/mL resin)