Thermo Scientific™ Pierce™ Recombinant Protein A Agarose, 25 mL

Thermo Scientific™ Pierce™ Recombinant Protein A Agarose, 25 mL

Thermo Scientific Pierce Recombinant Protein A Agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilized for use in IgG purification applications where the native ligand has undesirable effects.

The Pierce Recombinant Protein A Agarose uses a genetically engineered form of Protein A that is produced in E. coli and then immobilized on crosslinked 6% beaded agarose (CL-6B). Although some nonessential regions have been removed, recombinant is similar to native Protein A in overall size and IgG-binding domains. The protein has four high affinity binding sites capable of specifically binding to the Fc region of immunoglobulin molecules from several species.

Features

• Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG
• Standard capacity – loading of immobilized Protein A provides a binding capacity of 15 to 17 mg human IgG per milliliter of resin
• Agarose resin – crosslinked 6% beaded agarose for small column or batch affinity purification methods