Thermo Scientific™ Pierce™ Trypsin/Lys-C Protease Mix, MS-Grade, 20 µg

Thermo Scientific™ Pierce™ Trypsin/Lys-C Protease Mix, MS-Grade, 20 µg

Thermo Scientific Pierce Trypsin/Lys-C Protease Mix, MS-Grade, is a mass spectrometry (MS)-grade serine endoproteinase mixture of trypsin and LysC that can be used for concurrent digestion of proteins for more efficient digestion than with trypsin alone.

Protein characterization, identification, and quantification by MS begins with efficient, reproducible protein digestion. Although trypsin is routinely used for protein digestion, this protease alone is not sufficient to fully digest proteins at the carboxyl-end of lysine and arginine residues. Therefore, Lys-C protease is commonly combined with trypsin to sequentially digest proteins with fewer missed cleavages. Pierce Trypsin/Lys-C Protease Mix is a lyophilized mixture of trypsin and LysC proteases that has been optimized to improve digestion efficiency of proteins. It is provided in flexible formats of 20 µg, 5 x 20 µg, or 100 µg, and is also included in the EasyPep Mini MS Sample Prep Kit.

Features

• Enhanced digestion—enzyme combination reduces tryptic missed cleavages
• Convenient—trypsin and LysC proteases provided at an optimized ratio for digestion in a combined-use format
• Exceptional selectivity—trypsin has >95% C-terminal lysine and arginine specificity; LysC has >90% C-terminal lysine cleavage specificity
• Stable—enzyme mixture provided in a lyophilized format