Thermo Scientific™ Ponceau S Staining Solution

Thermo Scientific™ Ponceau S Staining Solution

Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is compatible with nitrocellulose and PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.

Ponceau S membrane staining is a nondestructive, reversible method to stain and detect proteins on nitrocellulose and PVDF membranes. The stain has minimal nonspecific interactions with the membrane surface and provides a reliable method for visualization of protein transfer to a membrane. The staining protocol is simple and quick and results in reddish-pink bands that are easily photographed. The stain can be reversed easily in less than five minutes. Subsequent western blot detection is unaffected as the stain does not alter the membrane-bound protein.