Thermo Scientific™ resDNASEQ™ Quantitative MDCK DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

Thermo Scientific™ resDNASEQ™ Quantitative MDCK DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.
This kit is bundled together with sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone without the sample preparation kit, please see resDNASEQ™ Quantitative MDCK DNA Kit.

Features

• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan™ primer/probe mix, and MDCK genomic DNA standard
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices

Contents

The DNA kit contains:

• TaqMan™ assay mix: 1 tube
• Master mix: 1 tube
• Quantitative genomic DNA standard: 1 tube
• DNA dilution buffer: 1 bottle
• Negative control water: 1 tube

The sample preparation kit contains:

• 1 box of buffers
• 1 box of magnetic beads
• 1 box of proteinase K enzyme
• 1 box contains tRNA, glycogen, proteinase K