Thermo Scientific™ SILAC Protein Quantitation Kit - DMEM:F12

Thermo Scientific™ SILAC Protein Quantitation Kit - DMEM:F12

Thermo Scientific SILAC Protein Quantitation Kit - DMEM:F12 is used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

Features

• Efficient—100% label incorporation into proteins of living cells
• Reproducible—eliminates intra-experimental variability caused by differential sample preparation
• Flexible—media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
• Compatible—label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others
• High-quality supplements—heavy amino acids with >99% isotope purity; dialyzed FBS tested to ensure that it is sterile, endotoxin-free, and cell culture compatible