Thermo Scientific™ SILAC Protein Quantitation Kit (Trypsin), DMEM

Thermo Scientific™ SILAC Protein Quantitation Kit (Trypsin), DMEM

The Thermo Scientific SILAC Protein Quantitation Kit (Trypsin), DMEM, is used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

Features

• Efficient—up to 100% label incorporation into proteins of living cells
• Reproducible—minimizes intra-experimental variability caused by differential sample preparation
• Flexible—media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
• Compatible—label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat, and others
• High-quality supplements—heavy amino acids with >99% isotope purity; dialyzed FBS tested to help ensure that it is sterile, endotoxin-free, and cell culture compatible

Contents

• DMEM for SILAC, 2 x 500 mL
• 13C6 15N2 L-lysine-2 HCl, 1 x 50 mg
• 13C6 15N4 L-arginine HCl, 1 x 50 mg
• L-lysine-2 HCl, 1 x 50 mg
• L-arginine-HCl, 1 x 50 mg, store at 4°C
• 100 mL FBS, store (at -20°C)