Thermo Scientific™ SulfoLink™ Coupling Resin, 50 mL

Thermo Scientific™ SulfoLink™ Coupling Resin, 50 mL

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

Features

• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 1 to 2 mg of peptide or 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin