Thermo Scientific™ SureQuant™ AKT Pathway (Phospho) IP and MS Sample Preparation Module
On demandThermo Scientific™ SureQuant™ AKT Pathway (Phospho) IP and MS Sample Preparation Module
The Thermo Scientific SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation for studies of key phosphorylation events of the AKT signaling pathway using mass spectrometry (MS).
The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This module is ideal for the multiplexed immuno-enrichment of key phosphorylated proteins in the AKT signaling pathway and has been optimized for use with the SureQuant quantitation modules.
For use with: Thermo Scientific™ Q Exactive Mass Spectrometer, Mass Spectrometer
Features
- Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
- Validated—antibodies validated for specificity and background
- Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
- Multiplex—able to immuno-enrich phosphorylated AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 in a single reaction
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