Thermo Scientific™ SureQuant™ AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)

Thermo Scientific™ SureQuant™ AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)

The Thermo Scientific SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of key phosphorylation events of AKT signaling pathway proteins using mass spectrometry (MS).

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key phosphorylated proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

For use with: Thermo Scientific™ Q Exactive Mass Spectrometer, Mass Spectrometer

Features

• Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
• Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
• Multiplex—able to quantitate phosphorylation of ten key proteins in the AKT signaling pathway
• Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

Contents

• SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module includes reagents necessary to immuno-enrich phosphorylated AKT-mTOR pathway proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
• SureQuant AKT Pathway (Phospho) Absolute Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide and LightPeptide mixtures for absolute peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation