Thermo Scientific™ T4 Polynucleotide Kinase (10 U/µL), 2500 Units
On demand
Order number :
EK0032
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/µL), 2500 Units
Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP, T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3'-phosphatase.
Features
- Active in Thermo Scientific restriction enzyme, RT, and T4 DNA ligase buffers
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