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Thermo Scientific™ T7 Gene 6 Exonuclease

On demand
Order number : 70025Z10KU
(Ex-work price)
$ 454

Thermo Scientific™ T7 Gene 6 Exonuclease


The T7 Gene 6 Exonuclease hydrolyzes duplex DNA non-processively in the 5'→3' direction from both 5'-phosphoryl or 5'-hydroxyl nucleotides by liberating oligonucleotides as well as mononucleotides, until about 50% of the DNA is acid soluble. It also degrades nucleotides at the gaps and nicks of double-stranded DNA from the 5'-termini and RNA from RNA:DNA hybrids in the 5'→3' direction.

The T7 Gene 6 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5' termini liberating 5' mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it will remove both 5'-hydroxyl and 5'-phosphoryl termini.

It also degrades RNA and DNA from RNA:DNA hybrids in the 5'→3' direction but is unable to degrade either double-stranded or single-stranded RNA.

This enzyme has been used for generating single-stranded DNA templates for sequencing or SNP analysis. 

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