Thermo Scientific™ TranscriptAid T7 High Yield Transcription Kit

Thermo Scientific™ TranscriptAid T7 High Yield Transcription Kit

Thermo Scientific TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 µL. Each reaction yields up to 200 µg RNA from 1 µg of template in 2 hours. The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA. All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Features

• Exceptionally high yields—up to 200 µg in 2 hours
• Versatile—suitable for both short and long RNA transcripts
• Milligram amounts of RNA in a single, scaled-up reaction
• Flexible—generates unlabeled, labeled or capped RNA
• RiboRuler RNA Ladder supplied with kit for sizing and quantification

Contents

• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
• ATP Solution
• CTP Solution
• GTP Solution
• UTP Solution
• Control template
• 3 M Sodium Acetate Solution, pH 5.2
• DEPC-treated Water
• 2X RNA Loading Dye
• RiboRuler High Range RNA Ladder, ready-to-use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol