Thermo Scientific™ UltraLink™ Biosupport, 1.25 g

Thermo Scientific™ UltraLink™ Biosupport, 1.25 g

Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.

Features

• High coupling efficiency and capacity—immobilizes proteins with very high efficiency and coupling capacity in 1 hour
• Specific and leak-proof coupling chemistry—reacts specifically with primary amines (-NH2), resulting in amide bonds that are stable for use in many affinity purification procedures; coupling reaction has no leaving group to contaminate samples
• Easy to use—no pre-swelling or secondary reagents required; simply weigh the needed amount of dry support and add the ligand solution to initiate coupling reaction
• Flexible coupling conditions—perform immobilization reaction in any of a variety of non-amine buffers and pH levels; coupling is most efficient in buffers containing a lyotropic salt such as sodium citrate; coupling compatible with or without organic solvent
• Excellent reusability—prepared affinity resin can be used with typical binding and elution procedures for over 100 cycles of affnity purification without significant loss of binding capacity
• Durable, high-performance resin—porous beads have a 60 µM diameter, can withstand 100 psi (6.9 bar) and allow for linear flow-through rates of 3000 cm/hour