Thermo Scientific™ UltraLink™ Hydrazide Resin

Thermo Scientific™ UltraLink™ Hydrazide Resin

Thermo Scientific UltraLink Hydrazide Resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

Features

• High-capacity—immobilize 1 to 10 mg of oxidized antibody or other glycoprotein per milliliter of resin
• Fast and efficient—immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)
• Specific immobilization—the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate
• Stable attachment—resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required
• Preserves antibody function—immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target