Thermo Scientific™ Yeast beta-Galactosidase Assay Kit

Thermo Scientific™ Yeast beta-Galactosidase Assay Kit

Thermo Scientific Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in-vivo using two-hybrid systems.

Features

• Efficient lysis of yeast cells and a colorimetric detection system
• Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
• Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
• Adaptable to 96-well microplates or tube assay formats
• Allows cells obtained from solid media or liquid culture to be assayed directly with no harvesting or washing steps.• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatible with prepared lysates from other species such as bacteria
• For Use With (Equipment): Microplate Reader

Contents

• Sufficient For: 250 colonies or 250 culture samples (100 μL each)
• Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)
• 2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)
• 1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)