Thermo Scientific™ 5-Fluoroorotic Acid, 5 g
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
5 g
Order number :
R0812
Thermo Scientific™ 5-Fluoroorotic Acid, 5 g
Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase. Yeasts with an active URA3 gene (Ura+) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).
Applications
- Detection of URA3 gene expression in yeast
- Construction of yeast 2-hybrid libraries
- Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutation
There are no report
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