Thermo Scientific™ GeneJET Stabilized and Fresh Whole Blood RNA Kit

Thermo Scientific™ GeneJET Stabilized and Fresh Whole Blood RNA Kit 50 preps

Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Features

•  Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
• Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
• Efficient—up to 4 µg of total RNA from 500 µL of healthy human blood
• Pure—isolates intact total RNA free of any RT-qPCR inhibitors
• Concentration: 2X

Contents

• Lysis Buffer for GeneJET Blood RNA Kit: 40 mL
• Wash Buffer 1 (conc.) for GeneJET Blood RNA Kit: 24 mL
• Wash Buffer 2 (conc.) for GeneJET Blood RNA Kit: 25 mL
• DNase l (lyophilized): 1 vial
• DNase I Reconstitution Buffer: 1 mL
• 2X DNase I Buffer: 4 mL
• Manganese Chloride Solution: 1 mL
• Water, nuclease-free: 30 mL
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes: 50
• Collection Tubes, 2 mL: 3 × 50
• Collection Tubes, 1.5 mL: 50