Thermo Scientific™ IPTG, dioxane-free, 5 g
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
5 g
Order number :
R0392
Thermo Scientific™ IPTG, dioxane-free, 5 g
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.
Applications
- Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (see Reference 1)
- nduction of gene expression under control of the lac promoter
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