Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR, 200

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR, 200

Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. The kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT. The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C).The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

Features

• High yields of full length cDNA up to 20 kb
• Efficient cDNA synthesis at wide temperature range from 42°C to 65°C
• Increased synthesis rate—complete cDNA synthesis in 15-30 minutes
• High sensitivity and specificity

Contents

• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix (reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers)
• Nuclease-free water