Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit, 200

Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit, 200

Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains pre-mixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features

• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in less than two hours
• Convenient—pre-mixed components
• Complete—includes all primers, controls and residual RNA removal reagents

Contents

• First Strand Enzyme Mix, 20 x 20 μL
• 4X First Strand Reaction Mix, 20 x 100 μL
• Second Strand Enzyme Mix, 20 x 60 μL
• 5X Second Strand Reaction Mix, 20 x 250 μL
• 0.5 M EDTA, pH 8.0 1 mL RNase I, 10 u/μL, 20 x 100 μL
• Control RNA, 0.5 μg/μL, 20 x 20 μL
• Oligo(dT)18 Primer, 100 μM, 0.5 μg/μL, 20 x 25 μL
• Random Hexamer Primer, 100 μM, 0.2 μg/μL, 20 x 25 μL
• Water, nuclease-free, 20 x 1.25 mL