Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit, 20
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K1651
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit, 20
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.
Features
- Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
- High yields of full-length first strand cDNA—with RNA templates up to 20 kb
- Flexible priming—oligo(dT)18, random hexamer, or gene-specific primers
Contents
- Maxima H Minus First Strand cDNA Synthesis Kit contains Maxima H Minus Enzyme Mix (includes Maxima H Minus Reverse Transcriptase and RiboLock RNase Inhibitor), Oligo(dT)18 and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water.
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