Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial, 1000
On demandThermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial, 1000
Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).
Features
- Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity—detects low copy number targets
- Wide linear range—accurate quantification across 9 orders of magnitude
- Reproducibility and convenience—ready-to-use 2x master mix
Contents
- 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 µL reactions
- 250 µL ROX solution, 50 µM
- 10 x 1.25 mL Nuclease-Free Water
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