Thermo Scientific™ Phire Green Hot Start II PCR Master Mix, 200

Thermo Scientific™ Phire Green Hot Start II PCR Master Mix, 200

Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction. The master mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

Features

• Quick hot start—No reactivation step
• Fast enzyme—Amplify four times faster than with hot start Taq
• Robust—Minimal reaction optimization due to high inhibitor tolerance
• High yields—Abundant products due to high efficiency
• Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
• Direct loading on gel

Contents

• 4 x 1.25 mL 2X Phire Green Hot Start II PCR Master Mix
• 1 x 0.5 mL DMSO
• 4 x 1.25 mL Water, nuclease free