Thermo Scientific™ Phusion U Green Hot Start DNA Polymerase, 500

Thermo Scientific™ Phusion U Green Hot Start DNA Polymerase, 500

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates. In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control. Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Features

• Accuracy—high fidelity DNA polymerase (25x Taq)
• Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
• Specificity—hot start for reduced nonspecific amplification and primer degradation
• Speed —short extension times (15-30 s/kb)
• Green format—permits direct loading of PCR products on gels

Contents

• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO