Thermo Scientific™ Phusion U Hot Start PCR Master Mix, 500
On demandThermo Scientific™ Phusion U Hot Start PCR Master Mix, 500
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates. In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control. Phusion U Hot Start PCR Master Mix is convenient 2X mix containing Phusion U Hot Start DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.
Features
- Accuracy—high fidelity DNA polymerase (25x Taq)
- Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
- Specificity—hot start for reduced nonspecific amplification and primer degradation
- Speed —short extension times (15-30 s/kb)
Contents
- Phusion U Hot Start DNA Polymerase
- 2X reaction buffer
- 3 mM MgCl2
- 400 µM of each dATP, dTTP, dCTP and dGTP
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