Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit

Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit

The Thermo Scientific Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.
This complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents.
The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with > 70% efficiency.

Features

• Non-radioactive—incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems
• Fast—RNA can be labeled in 0.5-2 hours with minimal downstream processing
• Easy to use—RNA ligase and optimized reaction buffer are included
• Economical—only a fraction of the cost of purchasing synthetic biotinylated RNA probes
• End-labeled—results in minimal disturbance of RNA secondary structure
• Flexible—label synthetic or in vitro transcribed RNA probes ranging from 22—450 nucleotides
  
  

Contents 

• T4 RNA Ligase Reaction Buffer (10X): 100 µL
• T4 RNA Ligase (20,000 U/mL): 40 µL
• Non-labeled RNA Control (10 µM): 100 µL
• Biotinylated IRE RNA Control (125 nM): 35 µL
• RNase Inhibitor (40 U/µL): 2 × 10 µL
• Nuclease-free Water: 1.5 mL
• DMSO: 200 µL
• 30% PEG: 300 µL
• Biotinylated Cytidine (Bis)phosphate (1mM): 40 µL
• Glycogen (20 mg/mL): 20 µL