Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/µL), 50000
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
5 x 10000 Units
Order number :
EP0442
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/µL), 50000
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features
- Efficient synthesis of full-length first strand cDNA up to 13 kb
- Optimum activity at 42°C
- Active up to 50°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Contents
- RevertAid Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT).
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