Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/µL)
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
500 Units
Order number :
EP0404
Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/µL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
Features
- Thermostable—half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4
- The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Contents
- 500 µL Taq DNA Polymerase (1 U/µL)
- 2 x 1.25 mL 10X Taq Buffer with KCl
- 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
- 2 x 1.25 mL 25 mM MgCl2
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