Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/µL), 100

Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/µL), 100

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

Features

• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Contains

• 20 µL Taq DNA Polymerase (5 U/µL)
• 0.6 mL 10X Taq Buffer with KCl
• 0.6 mL 10X Taq Buffer with (NH4)2SO4
• 0.6 mL 25 mM MgCl2