Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile

Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile

Uracil-DNA Glycosylase excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone. This cleavage generates alkali sensitive apyrimidinic sites that are blocked from replication by DNA polymerase or prevented from becoming a hybridization site.
Double and single-stranded dU-containing DNA are substrates for Uracil-DNA Glycosylase whereas RNA and normal dT-containing DNA are not.
Uracil-DNA Glycosylase can also be used to increase the cloning efficiency of PCR products having dU-containing primers incorporated into them as well as increasing the efficiency of site-directed mutagenesis.
The Uracil-DNA Glycosylase derived from Gadus morhua has all the attributes of the enzyme derived from E. coli with the added benefit of being heat-labile. It is completely and irreversibly inactivated after 10 minute incubation at 50°C.

Contents

• Uracil DNA Glycosylase 100 Units (1 U\ µL)