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Thermo Scientific™ X-Gal, 2 x 1 g

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 2 x 1 g
Order number : R0402
(Ex-work price)
$ On demand

Thermo Scientific™ X-Gal, 2 x 1 g

 

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies.

Applications

  • Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
  • Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells
  • Detection of beta-galactosidase activity in immunological and histochemical procedures
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