Thermo Scientific™ X-Gal, 2 x 1 g
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
2 x 1 g
Order number :
R0402
Thermo Scientific™ X-Gal, 2 x 1 g
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies.
Applications
- Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
- Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells
- Detection of beta-galactosidase activity in immunological and histochemical procedures