Thermo Scientific™ X-Gal Solution, ready-to-use
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
10 mL
Order number :
R0941
Thermo Scientific™ X-Gal Solution, ready-to-use
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies .
X-Gal Solution, ready-to-use, is stable, 0.22 µm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.
Applications
- Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
- Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells
- Detection of beta-galactosidase activity in immunological and histochemical procedures
There are no report
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