Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo MaxTaq DNA Polymerase
On demand
Brand origin :
Malaysia
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
100 reactions
Order number :
RTPL26
Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo MaxTaq DNA Polymerase
The 2-step RT-PCR kit is specially designed to provide reliable synthesis of full-length cDNA and convenient application of cDNA in PCR. M-MuLV RNase Hsynthesizes complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as the RNA strand does not degrade in DNA-RNA hybrid during first strand cDNA synthesis. With the variety of kit options for standard PCR and long PCR, the 2-step RT-PCR kit provides flexibility in an easy use format.
Contents
Viva cDNA Synthesis Kit
- 10X M-MuLV RT Buffer, 250µl.
- 10,000u M-MuLV Reverse Transcriptase.
- 10mM dNTPs mix, 0.25ml.
- RNase-free water 3ml.
- 40µM Oligo d( T ), 100µl.
- 50ng/µl Random hexamer, 100µl.
Viva PCR Kit
- 500u Chromo MaxTaq DNA Polymerase.
- 10X PCR Reaction Buffers.
- 50mM MgCl2.
- 10mM dNTPs Mix, 0.25ml.
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