Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual, 100 Reactions
On demandApplied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual, 100 Reactions
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
For use with equipments: Works on all AB platforms, but is optimized for the 7500.
Contents
Sufficient for 100 reactions of 25 µL, contains:
- 2 x RT-PCR Buffer: 1375 µL
- 25 x RT-PCR Enzyme Mix: 110 µL
- Nuclease-free Water: 1.75 mL
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