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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual, 100 Reactions

On demand
Order number : AM1005M
(Ex-work price)
$ On demand

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual, 100 Reactions


AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

• Recommended for RNA pathogen amplification

• Consistent amplification of RNA targets with high specificity and sensitivity

• Optimized to work with your target-specific primers and probes

• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

For use with equipments: Works on all AB platforms, but is optimized for the 7500.


Contents

Sufficient for 100 reactions of 25 µL, contains:

  • 2 x RT-PCR Buffer: 1375 µL 
  • 25 x RT-PCR Enzyme Mix: 110 µL 
  • Nuclease-free Water: 1.75 mL 


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