Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with PAXgene™ Blood RNA Tubes

Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with PAXgene™ Blood RNA Tubes

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in PAXgene® Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

Features

• Detect total RNA (including miRNA) from PAXgene® Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates.
• Sample Type: Blood
• Purification Target: Lysate, ready for real-time PCR

Contents

• Digestion Solution:  20 mL
• PAXgene™ Wash:  300 mL
• Stop Solution:  2 mL
• DNase 1:  200 µL