Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit, 200 Reactions

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

Features

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Contents

• 1 × 1 mL of 10X RT Buffer
• 1 × 1 mL of 10X RT Random Primers
• 1 × 0.2 mL of 25X dNTP Mix (100 mM)
• 2 × 0.1 mL of MultiScribe® Reverse Transcriptase (50 U/µL)