Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit, 400 Preps
On demandInvitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit, 400 Preps
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
This new addition to the family includes a Power SYBR™ Green 1-Step RT-PCR master mix. The 1-step format of the RT-PCR step streamlines the Cells-to- CT™ 'sample to answer' workflow more than ever without compromising sensitivity and specificity and at an extraordinary value.
For use with equipment: 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Features
- Complete—optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR™ Green 1-Step RT-PCR Master Mix
- Faster—7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
- Simple—12 steps total (sample prep and real-time RT-PCR combined)
- Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
- Affordable—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample prepared; study multiple targets per sample at an affordable price
Contents
- Lysis Solution, 22 mL, store at 2–8°C
- Dnase I, 220 µL, store at -5 to -30°C
- Stop Solution, 2 x 1 mL, store at -5 to -30°C
- Power SYBR™ Green 1-Step qPCR Mix, 20 mL, store at -5 to -30°C
- Power SYBR™ Green 1-Step RT Mix, 320 µL, store at -5 to -30°C
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