Applied Biosystems™ MAGnify™ Chromatin Immunoprecipitation System
On demand
Order number :
492024
Applied Biosystems™ MAGnify™ Chromatin Immunoprecipitation System
The MAGnify™ Chromatin Immunoprecipitation System provides a streamlined, optimized assay for the enrichment of chromatin/protein complexes and DNA recovery using magnetic bead capture technology. The isolated DNA is ready for downstream analysis by methods such as PCR- or qPCR-based assays, or massive parallel DNA sequencing.
Contents
Module 1 (shipped on wet ice, store at 4°C):
- 2 × 1 ml Glycine (1.25 M)
- 250 µl Dynabeads™ Protein A/G (do not freeze)
- 1.4 ml Reverse Crosslinking Buffer
- 500 µl DNA Purification Magnetic Beads (do not freeze)
- 1.4 ml DNA Purification Buffer
- 200 µl Proteinase K (20 mg/ml)
Module 2 (shipped on wet ice, store at 4°C):
- 10 ml IP Buffer 1
- 7.5 ml IP Buffer 2
- 8 ml DNA Wash Buffer
- 7.2 ml DNA Elution Buffer
Module 3 (shipped on dry ice, store at -20°C):
- 100 µl Protease Inhibitors (200X)
- 15 µl Mouse IgG (1 µg/µl)
- 15 µl Rabbit IgG (1 µg/µl)
Module (shipped on dry ice, store at -20°C):
- 8 ml Dilution Buffer
- 3.6 ml Lysis Buffer
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