Applied Biosystems™ PureQuant CD3+ T Cell Assay
On demand
Order number :
A47197
Applied Biosystems™ PureQuant CD3+ T Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples. Results are provided as a percentage of target cell type out of total input cells using a free analysis tool.
For use with equipment: QuantStudio™
Features
- Proven performance―core technology has been used for identification and quantification of immune cell types for over a decade
- Easily standardized―qPCR method enables consistent results across multiple users, instruments, and sites
- Facilitates regulatory filings―established performance specifications following ICHQ2(R1) guidelines
- Scheduling flexibility―assay can be performed using fresh or frozen cells/gDNA
Contents
- Dynabeads SILANE Genomic DNA Kit, store at 2–8°C
- 1 x 2 mL PureQuant qPCR Master Mix II, store at 2–8°C
- 4 x ABS
- THFA, store at room temperature
- PureQuant CD3 Module, store at -15 to -25°C (CD3 & GAPDH TaqMan primers and probes, Reference DNA, Lambda DNA, Standard DNA)
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